Fig. 9

Functional validation of real L1 ASP-associated genes in cancers. A CAGE-seq validation of L1 ASP-associated genes found 53 overlapping with the previously identified 903 L1 ASP-related genes. B KEGG pathway analysis suggested that real L1 ASP associated genes were primarily associated with GABAergic synapse pathway, cytokine-cytokine receptor interaction, chemokine signaling pathways, and exhibit certain connections with the classical PI3K Akt signaling pathway. C The real L1 ASP associated genes were significant up-regulation in cancer of LUSC and LUAD. D The GNGT1 gene was negatively correlated with disease-free survival in LUSC and LUAD patients. E The number of copies of the GNGT1 gene was significant up-regulation across various tumors