Description | Sequence (5′- > 3′) |
---|---|
Long adaptor with indexes | CAAGCAGAAGACGGCATACGAGATIndex1GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTIndex2*T |
Short adaptor with indexes | Index2AGATCGGAAGAGCGTCGTG |
1st round AluYb amplification primer | /5Biosg/CAGGCCGGACTGCGGA*C |
2nd round AluYb amplification primer | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNAGTGCTGGGATTACAGGCGTG*A |
1st round L1HS amplification primer | /5Biosg/GGGAGATATACCTAATGCTAGATGACAC*A |
 | /5Biosg/GGGAGATATACCTAATGCTAGATGACAC*G |
 | /5Biosg/GGGAGATATACCTAATGCTAGATGACAA*G |
2nd round L1HS amplification primer | TGCACATGTACCCTAAAACTTAGAGTATAA*T |
1st round SVA amplification primer | /5Biosg/AGAATCAGGCAGGGAGGTT*G |
2nd round SVA amplification primer | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNAGTACMGTCCAGCTTCGGC*T |
P7 adaptor amplification primer | CAAGCAGAAGACGGCATACGAGA*T |
L1_1 internal primer for validation | GGGAGATATACCTAATGCTAGATGACA |
L1_2 internal primer for validation | TGCACATGTACCCTAAAACTTAG |
SVA_1 internal primer for validation | AGAATCAGGCAGGGAGGTTG |
SVA_2 internal primer for validation | AGTACMGTCCAGCTTCGGCT |
/5Biosg/: 5′ Biotin; *: 3′ Phosphorothioate bond; Index1 and Index2: individual specific 6 bp indexes. |