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Fig. 8 | Mobile DNA

Fig. 8

From: Amplification of miniature inverted-repeat transposable elements and the associated impact on gene regulation and alternative splicing in mulberry (Morus notabilis)

Fig. 8

Alternative splicing of the MnPR-4 gene related to MITEs in different mulberry resources. a Genomic structure of MITE-related MnPR-4 splice variants. The arrowheads indicate primer positions for PCR amplification. b Site-specific PCR analysis using primers designed for the sequences flanking MnP4_scaffold205_783088–783,460. The primer positions are shown with the black arrows in a. c Detection of alternatively spliced transcripts. The reverse transcription PCR analysis of MnPR-4 expression in the leaf. The primer positions are shown with red arrows in a. The PCR products were separated by agarose gel electrophoresis, and the resulting gel was stained with ethidium bromide. PCR product sizes are indicated on the right. 1–4 represent M. notabilis; M. alba var. Taiwandaguo; M. alba var. Hongguo1 and water. M, marker

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Mobile DNA

ISSN: 1759-8753