Fig. 1
From: Long interspersed nuclear element-1 expression and retrotransposition in prostate cancer cells
Endogenous ORF1p and ORF2p in prostate cancer cells. a Western blot analysis of total protein lysates from the indicated prostate cancer cell lines using antibodies against LINE-1 proteins, ORF1 or ORF2, and tubulin. The numbers below the panels denote that densitometry of the band relative to tubulin. b Subcellular fractions (cytoplasmic, membrane, soluble nuclear, and chromatin bound) of LNCaP, DU-145, PC3, E006AA-hT, LAPC4, and 22RV1 prostate cancer cell lines were subjected to western blot analysis. The indicated antibodies were used. Controls for the fractionation include tubulin (cytoplasm), SP1 (soluble nuclear) and histone H3 (chromatin)