Table 1 Key Resources Table
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
Chemicals, Peptides, and Recombinant Proteins | ||
HinP1 | New England Biolabs | R0124S |
Phusion Polymerase | New England Biolabs | M0530S |
Critical Commercial Assays | ||
Wizard Genomic DNA Purification Kit | Promega | A1120 |
Quick Ligation Kit | New England Biolabs | M2200L |
Qiaquick PCR Cleanup Kit | Qiagen | 28,106 |
Axygen™ AxyPrep Mag™ PCR Clean-up Kits | Thermo Scientific | 14–223-227 |
Deposited Data | ||
Genomic Sequencing Data | This Study | https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/sra/PRJNA597349 |
Organisms/Strains | ||
MP1999 Mucts B::kan. Mu insertion between nts 3,652,046–3,652,051 | (Saha et al. 2013) [28] | ZL530 |
MG1655 with Mucts:cat at nt 4,203,381 (Ori-Mu) | (Walker et al. 2020) [29] | DMW11 |
MG1655 with Mucts:cat at nt 263,131 (OPR-Mu) | (Walker et al. 2020) [29] | DMW15 |
MG1655 with Mucts:cat at nt 3,339,450 (OPL-Mu) | (Walker et al. 2020) [29] | DMW22 |
MG1655 with Mucts:cat at nt 2,555,144 (TPL-Mu) | (Walker et al. 2020) [29] | DMW24 |
MG1655 with Mucts:cat at nt 792,226 (TPR-Mu) | (Walker et al. 2020) [29] | DMW33 |
MG1655 with Mucts:cat at nt 1,657,887 (Ter-Mu) | (Walker et al. 2020) [29] | DMW57 |
DMW57, Mucts:cat B::kan | This study, DMW57, ZL530 | DMW300 |
DMW33, Mucts:cat B::kan | This study, DMW33, ZL530 | DMW301 |
DMW24, Mucts:cat B::kan | This study, DMW24, ZL530 | DMW302 |
DMW22, Mucts:cat B::kan | This study, DMW22, ZL530 | DMW303 |
DMW15, Mucts:cat B::kan | This study, DMW15, ZL530 | DMW304 |
DMW11, Mucts:cat B::kan | This study, DMW11 ZL530 | DMW305 |
Software and Algorithms | ||
MAPS (Python) | This Study | |
BWA-MEM | Li H., 2013 | |