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Fig. 3 | Mobile DNA

Fig. 3

From: Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli

Fig. 3

MuB is responsible for capturing target sites near highly transcribed/translated genes. Twenty-two highly transcribed genes, plus the lac operon, flhD and dnaK-dnaJ, were selected for comparison between WT and ΔMuB insertion patterns. For WT, transpositions were pooled from all six prophage locations with an average of 5 million reads per prophage. ΔMuB experiments pooled all six prophages with an average of 20 million reads per prophage. Each gene is oriented to where the + 1 nt of the coding sequence (CDS) of the gene starts at the tick mark labeled + 1, and downstream sequence follow to the right. Upstream regions are marked by negatively labeled tick marks. The expected transcription start site labeled <TSS> is 125 nt away from the + 1 nt site. ΔMuB prophages showed an increase in an exclusion zone starting near the + 1 nt site in nearly every single case

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