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Fig. 2 | Mobile DNA

Fig. 2

From: Comparative analysis on the expression of L1 loci using various RNA-Seq preparations

Fig. 2

a Overlap of expressed L1 loci in cytoplasmic versus whole-cell RNA-Seq of prostate tumor cell line, 22Rv1. The 191 distinct L1 loci identified as expressed after manual curation (Fig. 1) were pooled in the two sets of cytoplasmic and whole-cell extracted RNA and then compared in a proportional Venn diagram [31]. In dark purple are the loci found in both whole-cell and cytoplasmic RNA of 22Rv1. In light purple are the loci found to-be-expressed in only the whole cell RNA-seq preparations of 22Rv1. In grey are the loci found to-be-expressed in only the cytoplasmic RNA-seq preparations of 22Rv1. The number of loci in each shaded region of the diagram is denoted. b Overlap of expressed L1 loci in cytoplasmic versus nuclear RNA-Seq of prostate tumor cell line, 22Rv1. The single matching set of cytoplasmic and nuclear extracted RNA were analyzed and manually curated to identify authentically expressed L1 loci. The 162 distinct L1 loci were compared in a proportional Venn diagram [31]. In dark green are the loci found to-be-expressed in both nuclear and cytoplasmic RNA of 22Rv1. In light green are the loci expressed in only the nuclear RNA-Seq of 22Rv1. In grey are the loci found in only the cytoplasmic RNA-Seq of 22Rv1. The number of loci in each shaded region of the diagram is denoted

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